ePBMC® Immunotyped PBMC

T Cell Reactivity Characterized PBMC

Our T Cell Reactivity Characterized ePBMC® have been characterized for peptide and protein antigen reactivity on the frequency of T cells that secrete IFN-, IL-2, IL-4, IL-5, and IL-17 in response to several Class I- and Class II-restricted antigens.

Available in ≥10 million cells per vial.

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Details

Our Cryopreserved ePBMC® are ideal for the following applications:

ELISPOT Assays
Tetramers/Pentamers
Intracytoplasmic Cytokine Staining (ICS)
Cytokine ELISA Assays
Cytokine Bead Arrays (CBA)
Cytokine Protein Arrays (CPA)
Cytokine mRNA Determinations (RT-PCR)
ADCC
Transmigration Assays
Any applications that require live, functional PBMC

Downloads

ePBMC® Brochure

CTL Serum-free Media Brochure

Protocols & Guidelines for Working with PBMC

Resting of cryopreserved PBMC does not generally benefit the performance of antigen-specific T cell ELISPOT Assays

Optimal thawing of cryopreserved PBMC for use in high-throughput human immune monitoring studies

ELISPOT assays in 384-well format allow analysis of 400 independent T cell tests with 10ml blood

ELISPOT assays provide reproducible results among different laboratories for T cell immune monitoring even in the hands of inexperienced investigators

Datasheets

Characterized ePBMC® Product Data Sheet

CTL-Test™ Medium	CTL-Wash™ Supplement 10x	CTL Anti-Aggregate Wash™ 20x Solution	Uncharacterized PBMC
HLA-typed Characterized PBMC	FcγRIIIa Polymorphism PBMC	NK-Activity PBMC	Reference Sample QC Set
ePBMC® Plasma Sample Sets