ePBMC® Plasma Sample Sets

In addition to PBMC, we also aliquot plasma from each donor. The presence of antibodies in this plasma can provide information that might help you select ePBMC® of your interest. CTL Plasma Sample Sets are typically screened for antibodies induced by vaccinations/infections and/or for autoantibodies.

When requesting a CTL Plasma Sample Set™, please indicate how many vials of the chosen plasma you wish to reserve. This way, we can be certain to select donors from our inventory that will meet or exceed the quantities you require. At that time, if you also wish to reserve matching PBMC, let us know and we'll reserve it for you.

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Our Cryopreserved ePBMC® are ideal for the following applications:

ELISPOT Assays
Tetramers/Pentamers
Intracytoplasmic Cytokine Staining (ICS)
Cytokine ELISA Assays
Cytokine Bead Arrays (CBA)
Cytokine Protein Arrays (CPA)
Cytokine mRNA Determinations (RT-PCR)
ADCC
Transmigration Assays
Any applications that require live, functional PBMC

Downloads

ePBMC® Brochure

CTL Serum-free Media Brochure

Protocols & Guidelines for Working with PBMC

Resting of cryopreserved PBMC does not generally benefit the performance of antigen-specific T cell ELISPOT Assays

Optimal thawing of cryopreserved PBMC for use in high-throughput human immune monitoring studies

ELISPOT assays in 384-well format allow analysis of 400 independent T cell tests with 10ml blood

ELISPOT assays provide reproducible results among different laboratories for T cell immune monitoring even in the hands of inexperienced investigators

Uncharacterized PBMC	T Cell Reactivity Characterized PBMC	HLA-typed Characterized PBMC	FcγRIIIa Polymorphism PBMC
NK-Activity PBMC	Reference Sample QC Set