Human Granzyme B/IgG/IgM Three-Color FluoroSpot

This optimized ELISPOT assay reliably detects human Granzyme B, IgG, and IgM secretions from total PBMC, isolated antibody-secreting cells that have been immunized/activated in vivo, or memory B cells that have been polyclonally stimulated in vitro to secret antibody.

This assay utilizes the Capture and Detection Kits listed below.

Available in 2 and 5 plate sets.

Components	Catalog #
Capture Kit	hCA3003F
Granzyme B Detection Kit	hT59
IgG Detection Kit	hB09
IgM Detection Kit	hB01

Scanning and analysis requires an analyzer with fluorescent capabilities. CTL recommends using a Series 6 Ultimate or an upgraded Series 6 Universal Analyzer running ImmunoSpot® Software version 5.3 or higher.

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Human Granzyme B/IgG/IgM Three-Color FluoroSpot

Details

ImmunoSpot® offers the ONLY COMPLETE kit which contains capture antibodies, fluorescently-labeled detection reagents, diluents, serum-free assay medium, polyclonal B cell activator (B-Poly-S™), and PVDF-membrane plates. Everything you need — all from ONE SOURCE!

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Protocol

Datasheets

SDS