Human IgG1/IgG2/IgG3 Three-Color FluoroSpot

This optimized FluoroSpot assay reliably detects and measures human IgG1, IgG2, and IgG3 secretions from either Antibody Secreting Cells (ASC) that have been immunized/activated in vivo, or memory B cells that have been polyclonally stimulated in vitro to secrete antibodies, in frequencies as low as one in a million.

This assay utilizes the Capture and Detection Kits listed below.

Available in 2 and 5 plate sets.

Components

Catalog #

 

Ig Capture Kit

hB3F

 

IgG1 Detection Kit

hB26

 

IgG2 Detection Kit

hB33

 

IgG3 Detection Kit

hB37

 
Scanning and analysis requires an analyzer with fluorescent capabilities. CTL recommends any of the following Series 5 or Series 6 readers running ImmunoSpot® Software version 5.3 or higher: S6UV, Ultra-V, Universal, Ultimate.



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Human IgG1/IgG2/IgG3 Three-Color FluoroSpotHuman IgG1/IgG2/IgG3 Three-Color FluoroSpot

Details

ImmunoSpot® offers the ONLY COMPLETE set of Kits which contain everything you need — ALL FROM ONE SOURCE!

Capture Kit
Includes Capture Antibodies, serum- free medium, low autofluorescence PVDF plates, polyclonal B cell activator (B-Poly-S™), and all diluents.

Detection Kit
Contains fluorescently-labeled Detection Antibodies and fluorochrome conjugates that bind to the secreted antibodies of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific Ig class/IgG subclass by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.

Datasheets

SDS

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