Human IgM/IgG/IgA/IgE Four-Color FluoroSpot

This optimized ELISPOT assay reliably detects human IgM, IgG, IgA, and IgE secretions from either Antibody Secreting Cells that have been immunized/activated in vivo, or memory B cells that have been polyclonally stimulated in vitro to secrete antibody.

This assay utilizes the Capture and Detection Kits listed below.

Available in 2 and 5 plate sets.


Catalog #


Ig Capture Kit



IgM Detection Kit



IgG Detection Kit



IgA Detection Kit



IgE Detection Kit


Scanning and analysis requires an analyzer with fluorescent capabilities. CTL recommends using a Series 6 Ultimate or an upgraded Series 6 Universal Analyzer running ImmunoSpot® Software version 5.3 or higher.

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Human IgM/IgG/IgA/IgE Four-Color FluoroSpotHuman IgM/IgG/IgA/IgE Four-Color FluoroSpot


ImmunoSpot® offers the ONLY COMPLETE set of Kits which contain everything you need — ALL FROM ONE SOURCE!

Capture Kit
Includes Capture Antibodies, serum- free medium, low autofluorescence PVDF plates, polyclonal B cell activator (B-Poly-S™), and all diluents.

Detection Kit
Contains fluorescently-labeled Detection Antibodies and fluorochrome conjugates that bind to the secreted antibodies of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific Ig class/IgG subclass by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.



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