Human IFN-γ/IL-2/IL-5/Granzyme B Four-Color FluoroSpot

This optimized FluoroSpot assay reliably detects and measures human IFN-γ, IL-2, IL-5 and Granzyme B secretions by stimulated effector cells in frequencies as low as one in a million.

This assay utilizes the Capture and Detection Kits listed below.

Available in 2 and 5 plate sets.

Components	Catalog #
IFN-γ/IL-2/IL-5/Granzyme B Capture Kit	hT4000F
IFN-γ Detection Kit	hT02
IL-2 Detection Kit	hT57
IL-5 Detection Kit	hT58
Granzyme B Detection Kit	hT59

Scanning and analysis requires an analyzer with fluorescent capabilities. CTL recommends using a Series 6 Ultimate or an upgraded Series 6 Universal Analyzer running ImmunoSpot® Software version 5.3 or higher.

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Human IFN-γ/IL-2/IL-5/Granzyme B Four-Color FluoroSpot

Details

ImmunoSpot® offers the ONLY COMPLETE set of Kits which contain everything you need — ALL FROM ONE SOURCE!

Capture Kit
Includes Capture Antibody for each cytokine plus serum-free medium, low autofluorescence PVDF plates, and all diluents.

Detection Kit
Contains fluorescently-labeled Detection Antibodies and fluorochrome conjugates that bind to the secreted cytokine of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific cytokine by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.