Mouse IFN-γ/TNF-α /IL-2 Triple-Color FluoroSpot

The ImmunoSpot IFN-γ/TNF-α/IL-2 assay is the first ever triple color mouse FluoroSpot

Detect cells secreting each cytokine individually or in combination(s) with accuracy comparable to single-color (single cytokine) analysis

Stimulated effector cells can be detected in frequencies as low as one in a million, allowing the enumeration of rare polyfunctional cells.

Analysis of the ImmunoSpot triple color FluoroSpot requires a fluorescence capable analyzer with the appropriate filters and light sources. CTL recommends any of the following Series 6 analyzers: Ultra-V, Universal, Ultimate. (ImmunoSpot v7.0 or higher software is recommended for accurate analysis).

This assay utilizes the Capture and Detection Kits listed below.
Available in 2 and 5 plate sets.

Components

Catalog #

IFN-γ/TNF-α/IL-2 Capture Kit

mT3001F

IFN-γ Detection Kit

mT01

TNF-α Detection Kit

mT13

IL-2 Detection Kit

mT17



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Mouse IFN-γ/TNF-α /IL-2 Triple-Color FluoroSpotMouse IFN-γ/TNF-α /IL-2 Triple-Color FluoroSpot

Details

ImmunoSpot® offers the ONLY COMPLETE set of Kits which contain everything you need — ALL FROM ONE SOURCE!

Capture Kit
Includes Capture Antibody for each cytokine plus serum-free medium, low autofluorescence PVDF plates, and all diluents.

Detection Kit
Contains fluorescently-labeled Detection Antibodies and fluorochrome conjugates that bind to the secreted cytokine of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific cytokine by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.

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