Mouse IFN-γ/TNF-α /IL-2 Triple-Color FluoroSpot

The ImmunoSpot IFN-γ/TNF-α/IL-2 assay is the first ever triple color mouse FluoroSpot

Detect cells secreting each cytokine individually or in combination(s) with accuracy comparable to single-color (single cytokine) analysis

Stimulated effector cells can be detected in frequencies as low as one in a million, allowing the enumeration of rare polyfunctional cells.

Analysis of the ImmunoSpot triple color FluoroSpot requires a fluorescence capable analyzer with the appropriate filters and light sources. CTL recommends any of the following Series 6 analyzers: Ultra-V, Universal, Ultimate. (ImmunoSpot v7.0 or higher software is recommended for accurate analysis).

This assay utilizes the Capture and Detection Kits listed below.
Available in 2 and 5 plate sets.

Components	Catalog #
IFN-γ/TNF-α/IL-2 Capture Kit	mT3001F
IFN-γ Detection Kit	mT01
TNF-α Detection Kit	mT13
IL-2 Detection Kit	mT17

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Mouse IFN-γ/TNF-α /IL-2 Triple-Color FluoroSpot

Details

ImmunoSpot® offers the ONLY COMPLETE set of Kits which contain everything you need — ALL FROM ONE SOURCE!

Capture Kit
Includes Capture Antibody for each cytokine plus serum-free medium, low autofluorescence PVDF plates, and all diluents.

Detection Kit
Contains fluorescently-labeled Detection Antibodies and fluorochrome conjugates that bind to the secreted cytokine of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific cytokine by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.