Mouse IgG2a/IgG2b Double-Color FluoroSpot

This optimized ELISPOT assay reliably detects mouse IgG2a and IgG2b secretions from either Antibody Secreting Cells that have been immunized/activated in vivo, or memory B cells that have been polyclonally stimulated in vitro to secrete antibody.

This assay utilizes the Capture and Detection Kits listed below.

Available in 2 and 5 plate sets in:

Components	Catalog #
Ig Capture Kit	mB2F
IgG2a Detection Kit	mB32
IgG2b Detection Kit	mB37

Scanning and analysis requires an analyzer with fluorescent capabilities. CTL recommends any of the following Series 5 or Series 6 readers running ImmunoSpot® Software version 5.3 or higher: S5UV, FluoroCore, Ultra, Universal, Ultimate.

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Mouse IgG2a/IgG2b Double-Color FluoroSpot

Details

ImmunoSpot® offers the ONLY COMPLETE set of Kits which contain everything you need — ALL FROM ONE SOURCE!

Capture Kit
Includes Capture Antibodies, serum- free medium, low autofluorescence PVDF plates, polyclonal B cell activator (B-Poly-S™), and all diluents.

Detection Kit
Contains fluorescently-labeled Detection Antibodies and fluorochrome conjugates that bind to the secreted antibodies of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific Ig class/IgG subclass by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.