Human Granzyme B/Perforin Double-Color FluoroSpot

This optimized FluoroSpot assay reliably detects and measures human Granzyme B and Perforin secretions by stimulated effector cells in frequencies as low as one in a million.

This assay utilizes the Capture and Detection Kits listed below.

 

Available in 2 and 5 plate sets.

 

Components

Catalog #

 

GzB/Perforin Capture Kit

hT2014F

 

GzB Detection Kit

hT08

 

Perforin Detection Kit

hT66

 

Scanning and analysis requires an analyzer with fluorescent capabilities. CTL recommends any of the following Series 5 or Series 6 readers running ImmunoSpot® Software version 5.3 or higher: S5UV, FluoroCore, Ultra V, Universal, Ultimate.



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Human Granzyme B/Perforin Double-Color FluoroSpotHuman Granzyme B/Perforin Double-Color FluoroSpot

Details

ImmunoSpot® offers the ONLY COMPLETE set of Kits which contain everything you need — ALL FROM ONE SOURCE!

Capture Kit
Includes Capture Antibody for each cytokine plus serum-free medium, low autofluorescence PVDF plates, and all diluents.

Detection Kit
Contains fluorescently-labeled Detection Antibodies and fluorochrome conjugates that bind to the secreted cytokine of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific cytokine by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.

Datasheets

SDS

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