Human IgM/IgA Double-Color FluoroSpot

This optimized ELISPOT assay reliably detects human IgM and IgA secretions from either Antibody Secreting Cells that have been immunized/activated in vivo, or memory B cells that have been polyclonally stimulated in vitro to secrete antibody.

This assay utilizes the Capture and Detection Kits listed below.

 

Available in 2 and 5 plate sets.

 

Components

Catalog #

 

Ig Capture Kit

hB2F

 

IgM Detection Kit

hB02

 

IgA Detection Kit

hB13

 

Scanning and analysis requires an analyzer with fluorescent capabilities. CTL recommends any of the following Series 5 or Series 6 readers running ImmunoSpot® Software version 5.3 or higher: S5UV, Ultra-V, Universal, Ultimate.



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Human IgM/IgA Double-Color FluoroSpotHuman IgM/IgA Double-Color FluoroSpot

Details

ImmunoSpot® offers the ONLY COMPLETE set of Kits which contain everything you need — ALL FROM ONE SOURCE!

Capture Kit
Includes Capture Antibodies, serum- free medium, low autofluorescence PVDF plates, polyclonal B cell activator (B-Poly-S™), and all diluents.

Detection Kit
Contains fluorescently-labeled Detection Antibodies and fluorochrome conjugates that bind to the secreted antibodies of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific Ig class/IgG subclass by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.

Datasheets

SDS

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